HEADER RIBONUCLEIC ACID 18-AUG-88 ARB004 TITLE RNA DOUBLE-HELICAL FRAGMENTS AT ATOMIC RESOLUTION: II. THE TITLE 2 CRYSTAL STRUCTURE OF SODIUM GUANYLYL-3',5'-CYTIDINE TITLE 3 NONHYDRATE COMPND MOL_ID: 1; COMPND 2 MOLECULE: RNA (5'-R(*GP*C)-3'); COMPND 3 CHAIN: A; COMPND 4 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 SYNTHETIC: YES KEYWDS A-RNA, DOUBLE HELIX EXPDTA X-RAY DIFFRACTION AUTHOR J.M.ROSENBERG,N.C.SEEMAN,R.O.DAY,A.RICH REVDAT 2 21-SEP-01 5 REVDAT 1 18-AUG-88 0 JRNL AUTH J.M.ROSENBERG,N.C.SEEMAN,R.O.DAY,A.RICH JRNL TITL RNA DOUBLE-HELICAL FRAGMENTS AT ATOMIC RESOLUTION: JRNL TITL 2 II. THE CRYSTAL STRUCTURE OF SODIUM JRNL TITL 3 GUANYLYL-3',5'-CYTIDINE NONHYDRATE JRNL REF J.MOL.BIOL. V. 104 145 1976 JRNL REFN ASTM JMOBAK UK ISSN 0022-2836 REMARK 1 REMARK 1 REFERENCE 1 REMARK 1 AUTH R.O.DAY,N.C.SEEMAN,J.M.ROSENBERG,A.RICH REMARK 1 TITL A CRYSTALLINE FRAGMENT OF THE DOUBLE HELIX: THE REMARK 1 TITL 2 STRUCTURE OF THE DINUCLEOSIDE PHOSPHATE REMARK 1 TITL 3 GUANYLYL-3',5'-CYTIDINE REMARK 1 REF PROC.NAT.ACAD.SCI.USA V. 70 849 1973 REMARK 1 REFN ASTM PNASA6 US ISSN 0027-8424 REMARK 2 REMARK 2 RESOLUTION. 0.89 ANGSTROM. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 REMARK 3 DATA USED IN REFINEMENT. REMARK 3 RESOLUTION RANGE HIGH (ANGSTROMS) : 0.89 REMARK 3 RESOLUTION RANGE LOW (ANGSTROMS) : NULL REMARK 3 DATA CUTOFF (SIGMA(F)) : 3.000 REMARK 3 DATA CUTOFF HIGH (ABS(F)) : NULL REMARK 3 DATA CUTOFF LOW (ABS(F)) : NULL REMARK 3 COMPLETENESS (WORKING+TEST) (%) : NULL REMARK 3 NUMBER OF REFLECTIONS : 2276 REMARK 3 REMARK 3 REMARK 3 FIT TO DATA USED IN REFINEMENT. REMARK 3 CROSS-VALIDATION METHOD : NULL REMARK 3 FREE R VALUE TEST SET SELECTION : NULL REMARK 3 R VALUE (WORKING SET) : 0.054 REMARK 3 FREE R VALUE : NULL REMARK 3 FREE R VALUE TEST SET SIZE (%) : NULL REMARK 3 FREE R VALUE TEST SET COUNT : NULL REMARK 3 ESTIMATED ERROR OF FREE R VALUE : NULL REMARK 3 REMARK 3 FIT IN THE HIGHEST RESOLUTION BIN. REMARK 3 TOTAL NUMBER OF BINS USED : NULL REMARK 3 BIN RESOLUTION RANGE HIGH (A) : NULL REMARK 3 BIN RESOLUTION RANGE LOW (A) : NULL REMARK 3 BIN COMPLETENESS (WORKING+TEST) (%) : NULL REMARK 3 REFLECTIONS IN BIN (WORKING SET) : NULL REMARK 3 BIN R VALUE (WORKING SET) : NULL REMARK 3 BIN FREE R VALUE : NULL REMARK 3 BIN FREE R VALUE TEST SET SIZE (%) : NULL REMARK 3 BIN FREE R VALUE TEST SET COUNT : NULL REMARK 3 ESTIMATED ERROR OF BIN FREE R VALUE : NULL REMARK 3 REMARK 3 NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT. REMARK 3 PROTEIN ATOMS : 0 REMARK 3 NUCLEIC ACID ATOMS : 40 REMARK 3 HETEROGEN ATOMS : 1 REMARK 3 SOLVENT ATOMS : 10 REMARK 3 REMARK 3 B VALUES. REMARK 3 FROM WILSON PLOT (A**2) : NULL REMARK 3 MEAN B VALUE (OVERALL, A**2) : NULL REMARK 3 OVERALL ANISOTROPIC B VALUE. REMARK 3 B11 (A**2) : NULL REMARK 3 B22 (A**2) : NULL REMARK 3 B33 (A**2) : NULL REMARK 3 B12 (A**2) : NULL REMARK 3 B13 (A**2) : NULL REMARK 3 B23 (A**2) : NULL REMARK 3 REMARK 3 ESTIMATED COORDINATE ERROR. REMARK 3 ESD FROM LUZZATI PLOT (A) : NULL REMARK 3 ESD FROM SIGMAA (A) : NULL REMARK 3 LOW RESOLUTION CUTOFF (A) : NULL REMARK 3 REMARK 3 CROSS-VALIDATED ESTIMATED COORDINATE ERROR. REMARK 3 ESD FROM C-V LUZZATI PLOT (A) : NULL REMARK 3 ESD FROM C-V SIGMAA (A) : NULL REMARK 3 REMARK 3 RMS DEVIATIONS FROM IDEAL VALUES. REMARK 3 BOND LENGTHS (A) : NULL REMARK 3 BOND ANGLES (DEGREES) : NULL REMARK 3 DIHEDRAL ANGLES (DEGREES) : NULL REMARK 3 IMPROPER ANGLES (DEGREES) : NULL REMARK 3 REMARK 3 ISOTROPIC THERMAL MODEL : NULL REMARK 3 REMARK 3 ISOTROPIC THERMAL FACTOR RESTRAINTS. RMS SIGMA REMARK 3 MAIN-CHAIN BOND (A**2) : NULL ; NULL REMARK 3 MAIN-CHAIN ANGLE (A**2) : NULL ; NULL REMARK 3 SIDE-CHAIN BOND (A**2) : NULL ; NULL REMARK 3 SIDE-CHAIN ANGLE (A**2) : NULL ; NULL REMARK 3 REMARK 3 REMARK 3 NCS MODEL : NULL REMARK 3 REMARK 3 NCS RESTRAINTS. RMS SIGMA/WEIGHT REMARK 3 GROUP 1 POSITIONAL (A) : NULL ; NULL REMARK 3 GROUP 1 B-FACTOR (A**2) : NULL ; NULL REMARK 3 REMARK 3 PARAMETER FILE 1 : NULL REMARK 3 TOPOLOGY FILE 1 : NULL REMARK 3 REMARK 3 OTHER REFINEMENT REMARKS: NULL REMARK 4 REMARK 4 NULL COMPLIES WITH FORMAT V. 2.3, 09-JULY-1998 REMARK 105 REMARK 105 THE PROTEIN DATA BANK HAS ADOPTED THE SACCHARIDE CHEMISTS REMARK 105 NOMENCLATURE FOR ATOMS OF THE DEOXYRIBOSE/RIBOSE MOIETY REMARK 105 RATHER THAN THAT OF THE NUCLEOSIDE CHEMISTS. THE RING REMARK 105 OXYGEN ATOM IS LABELLED O4* INSTEAD OF O1*. REMARK 106 REMARK 106 ALL HYDROGEN BONDS BETWEEN BASE PAIRS NOT MENTIONED IN REMARK 106 REMARKS 102 AND 103 FOLLOW THE CONVENTIONAL WATSON-CRICK REMARK 106 HYDROGEN BONDING PATTERN. THEY HAVE NOT BEEN PRESENTED ON REMARK 106 *CONECT* RECORDS IN THIS ENTRY. REMARK 200 REMARK 200 EXPERIMENTAL DETAILS REMARK 200 EXPERIMENT TYPE : X-RAY DIFFRACTION REMARK 200 DATE OF DATA COLLECTION : NULL REMARK 200 TEMPERATURE (KELVIN) : 283.0 REMARK 200 PH : NULL REMARK 200 NUMBER OF CRYSTALS USED : NULL REMARK 200 REMARK 200 SYNCHROTRON (Y/N) : N REMARK 200 RADIATION SOURCE : NULL REMARK 200 BEAMLINE : NULL REMARK 200 X-RAY GENERATOR MODEL : NULL REMARK 200 MONOCHROMATIC OR LAUE (M/L) : M REMARK 200 WAVELENGTH OR RANGE (A) : 1.5400 REMARK 200 MONOCHROMATOR : NULL REMARK 200 OPTICS : NULL REMARK 200 REMARK 200 DETECTOR TYPE : DIFFRACTOMETER REMARK 200 DETECTOR MANUFACTURER : SYNTEX PT REMARK 200 INTENSITY-INTEGRATION SOFTWARE : NULL REMARK 200 DATA SCALING SOFTWARE : NULL REMARK 200 REMARK 200 NUMBER OF UNIQUE REFLECTIONS : 2276 REMARK 200 RESOLUTION RANGE HIGH (A) : 0.890 REMARK 200 RESOLUTION RANGE LOW (A) : NULL REMARK 200 REJECTION CRITERIA (SIGMA(I)) : NULL REMARK 200 REMARK 200 OVERALL. REMARK 200 COMPLETENESS FOR RANGE (%) : NULL REMARK 200 DATA REDUNDANCY : NULL REMARK 200 R MERGE (I) : NULL REMARK 200 R SYM (I) : NULL REMARK 200 FOR THE DATA SET : NULL REMARK 200 REMARK 200 IN THE HIGHEST RESOLUTION SHELL. REMARK 200 HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : NULL REMARK 200 HIGHEST RESOLUTION SHELL, RANGE LOW (A) : NULL REMARK 200 COMPLETENESS FOR SHELL (%) : NULL REMARK 200 DATA REDUNDANCY IN SHELL : NULL REMARK 200 R MERGE FOR SHELL (I) : NULL REMARK 200 R SYM FOR SHELL (I) : NULL REMARK 200 FOR SHELL : NULL REMARK 200 REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: NULL REMARK 200 SOFTWARE USED: NULL REMARK 200 STARTING MODEL: NULL REMARK 200 REMARK 200 REMARK: NULL REMARK 280 REMARK 280 CRYSTAL REMARK 280 SOLVENT CONTENT, VS (%): NULL REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): NULL REMARK 280 REMARK 280 CRYSTALLIZATION CONDITIONS: MPD, NA CACODYLATE, 9-NH2- REMARK 280 ACRIDINE_HCL REMARK 290 REMARK 290 CRYSTALLOGRAPHIC SYMMETRY REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1 REMARK 290 REMARK 290 SYMOP SYMMETRY REMARK 290 NNNMMM OPERATOR REMARK 290 1555 X,Y,Z REMARK 290 2555 -X,Y,-Z REMARK 290 3555 1/2+X,1/2+Y,Z REMARK 290 4555 1/2-X,1/2+Y,-Z REMARK 290 REMARK 290 WHERE NNN -> OPERATOR NUMBER REMARK 290 MMM -> TRANSLATION VECTOR REMARK 290 REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY REMARK 290 RELATED MOLECULES. REMARK 290 SMTRY1 1 1.000000 0.000000 0.000000 0.00000 REMARK 290 SMTRY2 1 0.000000 1.000000 0.000000 0.00000 REMARK 290 SMTRY3 1 0.000000 0.000000 1.000000 0.00000 REMARK 290 SMTRY1 2 -1.000000 0.000000 0.000000 0.00000 REMARK 290 SMTRY2 2 0.000000 1.000000 0.000000 0.00000 REMARK 290 SMTRY3 2 0.000000 0.000000 -1.000000 0.00000 REMARK 290 SMTRY1 3 1.000000 0.000000 0.000000 10.73000 REMARK 290 SMTRY2 3 0.000000 1.000000 0.000000 8.14850 REMARK 290 SMTRY3 3 0.000000 0.000000 1.000000 0.00000 REMARK 290 SMTRY1 4 -1.000000 0.000000 0.000000 10.73000 REMARK 290 SMTRY2 4 0.000000 1.000000 0.000000 8.14850 REMARK 290 SMTRY3 4 0.000000 0.000000 -1.000000 0.00000 REMARK 290 REMARK 290 REMARK: NULL REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT REMARK 300 WHICH CONSISTS OF 1 CHAIN(S). SEE REMARK 350 FOR REMARK 300 INFORMATION ON GENERATING THE BIOLOGICAL MOLECULE(S). REMARK 350 REMARK 350 GENERATING THE BIOMOLECULE REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 APPLY THE FOLLOWING TO CHAINS: A REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 350 BIOMT1 2 -1.000000 0.000000 0.000000 21.37205 REMARK 350 BIOMT2 2 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 2 0.000000 0.000000 -1.000000 9.33159 REMARK 375 REMARK 375 SPECIAL POSITION REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL REMARK 375 POSITIONS. REMARK 375 REMARK 375 ATOM RES CSSEQI REMARK 375 HOH A 4 LIES ON A SPECIAL POSITION. REMARK 375 HOH A 13 LIES ON A SPECIAL POSITION. SEQRES 1 A 2 G C HET NA A 3 1 HETNAM NA SODIUM ION FORMUL 2 NA NA1 1+ FORMUL 3 HOH *10(H2 O1) CRYST1 21.460 16.297 9.332 90.00 90.54 90.00 C 1 2 1 4 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 0.046598 0.000000 0.000439 0.00000 SCALE2 0.000000 0.061361 0.000000 0.00000 SCALE3 0.000000 0.000000 0.107163 0.00000 ATOM 1 O5* G A 1 4.787 6.423 -1.333 1.00 10.00 O ANISOU 1 O5* G A 1 5400 4100 8600 0 -600 1800 O ATOM 2 C5* G A 1 4.382 5.500 -2.314 1.00 10.00 C ANISOU 2 C5* G A 1 3800 6200 5600 -600 -500 2300 C ATOM 3 C4* G A 1 4.944 4.200 -1.989 1.00 10.00 C ANISOU 3 C4* G A 1 2700 4600 3900 -100 -100 1100 C ATOM 4 O4* G A 1 6.394 4.195 -2.141 1.00 10.00 O ANISOU 4 O4* G A 1 2500 6000 4200 -200 -300 1700 O ATOM 5 C3* G A 1 4.741 3.742 -0.559 1.00 10.00 C ANISOU 5 C3* G A 1 2500 3200 3300 -600 -100 300 C ATOM 6 O3* G A 1 3.440 3.178 -0.447 1.00 10.00 O ANISOU 6 O3* G A 1 2600 4000 3300 -1100 -100 100 O ATOM 7 C2* G A 1 5.879 2.769 -0.332 1.00 10.00 C ANISOU 7 C2* G A 1 2500 3300 3500 -300 -500 300 C ATOM 8 O2* G A 1 5.550 1.582 -0.946 1.00 10.00 O ANISOU 8 O2* G A 1 3900 3000 4800 -200 -800 -900 O ATOM 9 C1* G A 1 7.000 3.460 -1.128 1.00 10.00 C ANISOU 9 C1* G A 1 2300 4300 3500 300 -400 300 C ATOM 10 N9 G A 1 7.764 4.353 -0.250 1.00 10.00 N ANISOU 10 N9 G A 1 2000 3500 4500 -400 -800 400 N ATOM 11 C8 G A 1 7.704 5.662 -0.211 1.00 10.00 C ANISOU 11 C8 G A 1 3700 3700 7000 -400 -2000 1100 C ATOM 12 N7 G A 1 8.559 6.191 0.630 1.00 10.00 N ANISOU 12 N7 G A 1 4300 3600 6900 -400 -2100 800 N ATOM 13 C5 G A 1 9.252 5.125 1.127 1.00 10.00 C ANISOU 13 C5 G A 1 3400 2800 4400 -200 0 500 C ATOM 14 C6 G A 1 10.348 5.075 2.077 1.00 10.00 C ANISOU 14 C6 G A 1 2800 3800 5300 -800 -500 700 C ATOM 15 O6 G A 1 10.929 5.961 2.598 1.00 10.00 O ANISOU 15 O6 G A 1 4600 3600 7900 -1100 -2800 200 O ATOM 16 N1 G A 1 10.730 3.812 2.329 1.00 10.00 N ANISOU 16 N1 G A 1 3200 2900 4600 -400 -300 300 N ATOM 17 C2 G A 1 10.162 2.718 1.782 1.00 10.00 C ANISOU 17 C2 G A 1 3100 4000 4500 100 -400 100 C ATOM 18 N2 G A 1 10.637 1.576 2.153 1.00 10.00 N ANISOU 18 N2 G A 1 5200 3500 7300 500 -2700 600 N ATOM 19 N3 G A 1 9.147 2.780 0.876 1.00 10.00 N ANISOU 19 N3 G A 1 3100 3400 4200 200 -900 -500 N ATOM 20 C4 G A 1 8.771 4.004 0.619 1.00 10.00 C ANISOU 20 C4 G A 1 2500 3600 3500 200 0 700 C ATOM 21 P C A 2 2.635 3.259 0.945 1.00 10.00 P ANISOU 21 P C A 2 2200 3100 3000 -100 -300 100 P ATOM 22 O1P C A 2 1.312 2.669 0.630 1.00 10.00 O ANISOU 22 O1P C A 2 2400 7200 3600 -1100 -500 700 O ATOM 23 O2P C A 2 2.716 4.550 1.506 1.00 10.00 O ANISOU 23 O2P C A 2 4300 4100 5500 300 900 100 O ATOM 24 O5* C A 2 3.469 2.361 1.940 1.00 10.00 O ANISOU 24 O5* C A 2 3400 3500 3800 -800 -1200 200 O ATOM 25 C5* C A 2 3.403 0.973 1.842 1.00 10.00 C ANISOU 25 C5* C A 2 3400 3200 2800 -700 -600 -100 C ATOM 26 C4* C A 2 4.206 0.398 2.976 1.00 10.00 C ANISOU 26 C4* C A 2 3200 2400 3300 100 0 -300 C ATOM 27 O4* C A 2 5.599 0.699 2.762 1.00 10.00 O ANISOU 27 O4* C A 2 2800 4300 3500 0 -300 -600 O ATOM 28 C3* C A 2 3.910 0.901 4.361 1.00 10.00 C ANISOU 28 C3* C A 2 4300 2800 3300 -800 0 200 C ATOM 29 O3* C A 2 2.693 0.337 4.841 1.00 10.00 O ANISOU 29 O3* C A 2 4800 4800 4000 -1500 500 -100 O ATOM 30 C2* C A 2 5.180 0.515 5.107 1.00 10.00 C ANISOU 30 C2* C A 2 5800 3100 3500 -600 -1200 -100 C ATOM 31 O2* C A 2 5.145 -0.805 5.356 1.00 10.00 O ANISOU 31 O2* C A 2 7900 3200 5400 -1300 -2200 1300 O ATOM 32 C1* C A 2 6.250 0.807 4.030 1.00 10.00 C ANISOU 32 C1* C A 2 4100 2600 3900 700 -1600 -900 C ATOM 33 N1 C A 2 6.809 2.135 4.137 1.00 10.00 N ANISOU 33 N1 C A 2 3700 2600 4000 -300 -900 -100 N ATOM 34 C2 C A 2 7.941 2.308 4.929 1.00 10.00 C ANISOU 34 C2 C A 2 3000 3600 3800 200 -300 -400 C ATOM 35 O2 C A 2 8.434 1.359 5.492 1.00 10.00 O ANISOU 35 O2 C A 2 4600 2900 7400 500 -2900 200 O ATOM 36 N3 C A 2 8.446 3.502 5.064 1.00 10.00 N ANISOU 36 N3 C A 2 3700 1900 4300 300 -100 -300 N ATOM 37 C4 C A 2 7.855 4.514 4.536 1.00 10.00 C ANISOU 37 C4 C A 2 3000 4000 2700 -100 600 -200 C ATOM 38 N4 C A 2 8.312 5.694 4.766 1.00 10.00 N ANISOU 38 N4 C A 2 4800 3000 5700 -400 -900 -600 N ATOM 39 C5 C A 2 6.682 4.363 3.708 1.00 10.00 C ANISOU 39 C5 C A 2 4300 2800 3900 800 -700 700 C ATOM 40 C6 C A 2 6.214 3.163 3.549 1.00 10.00 C ANISOU 40 C6 C A 2 3700 3500 3800 400 500 100 C TER 41 C A 2 HETATM 42 NA NA A 3 0.544 1.812 7.848 1.00 10.00 NA ANISOU 42 NA NA A 3 3300 4300 4200 400 400 -500 NA HETATM 43 O HOH A 4 10.730 8.122 0.000 1.00 10.00 O ANISOU 43 O HOH A 4 5300 2900 4500 0 900 0 O HETATM 44 O HOH A 5 0.193 0.424 3.172 1.00 10.00 O ANISOU 44 O HOH A 5 4800 5000 5000 400 -600 500 O HETATM 45 O HOH A 6 2.660 0.585 7.584 1.00 10.00 O ANISOU 45 O HOH A 6 3700 4300 3800 0 300 -300 O HETATM 46 O HOH A 7 0.944 3.356 6.178 1.00 10.00 O ANISOU 46 O HOH A 7 14400 22800 22700 -3500 100 16000 O HETATM 47 O HOH A 8 2.785 8.569 9.375 1.00 10.00 O ANISOU 47 O HOH A 8 6400 5300 11900 100 -3200 -500 O HETATM 48 O HOH A 9 3.800 6.773 2.090 1.00 10.00 O ANISOU 48 O HOH A 9 56800 11800 23900 -19900 -31000 10400 O HETATM 49 O HOH A 10 1.252 7.148 7.665 1.00 10.00 O ANISOU 49 O HOH A 10 12800 7400 13500 600 -4200 -1200 O HETATM 50 O HOH A 11 1.321 7.047 4.841 1.00 10.00 O ANISOU 50 O HOH A 11 7700 7500 12400 -1800 -1500 600 O HETATM 51 O HOH A 12 2.220 4.594 4.265 1.00 10.00 O ANISOU 51 O HOH A 12 50800 11300 11800 0 18200 -700 O HETATM 52 O HOH A 13 0.000 5.339 0.000 1.00 10.00 O ANISOU 52 O HOH A 13 11700 6700 41800 0 4000 0 O MASTER 225 0 1 0 0 0 0 6 51 1 0 1 END